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KMID : 0616120230540020066
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Korean Journal of Pharmacognosy
2023 Volume.54 No. 2 p.66 ~ p.71
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Antioxidant Activity of Ethyl acetate Fraction of Berberis koreana Palibin in Caenorhabditis elegans
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Choi Ji-Woo
Kim Jun-Hyeong
Lee Jae-Hyeok
Kim Dae-Keun
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Abstract
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Ethyl acetate (EA) soluble fraction of the Berberis amurensis (Berberidaceae) methanol extract showed the potent DPPH radical scavenging activity through Caenorhabditis elegans model system. The EA fraction was measured for the activity of superoxide dismutase (SOD), catalase, and oxidative stress tolerance by using C. elegans along with reactive oxygen species (ROS) level. In addition, SOD-3 expression was conducted using a transgenic strain (CF1553) to confirm that the regulation of the stress response gene is responsible for the increased stress tolerance of C. elegans treated by the EA fraction. As a result, the EA soluble fraction of B. amurensis increased SOD and catalase activity, and decreased ROS accumulation in a dose-dependent manner. Furthermore, the EA fraction-treated CF1553 worm showed higher SOD-3::GFP intensity than the control worm.
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KEYWORD
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Berberis koreana, Caenorhabditis elegans, Antioxidant activity, ROS, SOD
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